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Related Experiment Video

Updated: May 18, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
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Detection of Vancomycin-Resistant Enterococcus Spp. (VRE) from Poultry.

H Toosa1, S Radu, G Rusul

  • 1Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia 43400, Serdang, Selangor D.E., Malaysia.

The Malaysian Journal of Medical Sciences : MJMS
|September 14, 2012
PubMed
Summary

Chicken samples revealed high levels of antibiotic resistance in Enterococcus species, including vancomycin resistance. This indicates significant contamination risks in food markets, necessitating further investigation and control measures.

Keywords:
Enterococcus spp.plasmidpoultryvancomycin-resistant

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Published on: February 1, 2014

Area of Science:

  • Veterinary Microbiology
  • Food Safety
  • Antimicrobial Resistance

Background:

  • Enterococcus species are common bacteria found in food products.
  • Antibiotic resistance in foodborne pathogens poses a significant public health concern.
  • Previous studies have indicated the presence of resistant Enterococcus in food animals.

Purpose of the Study:

  • To investigate the prevalence of antibiotic resistance in Enterococcus faecalis and Enterococcus hirae isolated from chicken samples.
  • To determine the susceptibility of these isolates to vancomycin and other common antimicrobial agents.
  • To assess the level of contamination risk based on multiple antibiotic resistance.

Main Methods:

  • Isolation and identification of Enterococcus species from chicken samples.
  • Antimicrobial susceptibility testing using various antibiotics, including vancomycin.
  • Calculation of the multiple antibiotic resistance index (MARI).
  • Plasmid profile analysis to investigate potential genetic determinants of resistance.

Main Results:

  • All Enterococcus isolates exhibited resistance to multiple antibiotics, with 100% resistance to ceftaxidime, cephalothin, erythromycin, gentamicin, kanamycin, nalidixic acid, and streptomycin.
  • High vancomycin resistance rates were observed in both Enterococcus faecalis (27/28) and Enterococcus hirae (4/5) isolates.
  • Multiple antibiotic resistance index values ranged from 0.64 to 1.0, suggesting high-level contamination.
  • Plasmid DNA was detected in some isolates, but no correlation was found between plasmid patterns and antibiotic resistance.

Conclusions:

  • Chicken samples from markets in Sri Serdang harbor Enterococcus species with extensive multidrug resistance, including to vancomycin.
  • The high MARI values indicate a significant risk of contamination, potentially linked to the food supply chain.
  • Further research is needed to understand the mechanisms of resistance and to implement effective control strategies to mitigate public health risks.