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Updated: May 18, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
AT_CHLORO: A Chloroplast Protein Database Dedicated to Sub-Plastidial Localization
Christophe Bruley1, Véronique Dupierris, Daniel Salvi
1CEA, DSV, IRTSV, Laboratoire Biologie à Grande Echelle, Institut de Recherches en Technologie et Sciences pour le Vivant Grenoble, France.
Abstract:
AT_CHLORO (www.grenoble.prabi.fr/at_chloro) is a database dedicated to sub-plastidial localization of A. thaliana chloroplast proteins. This information was infered from proteomics experiments obtained from a comprehensive study that allowed the identification of proteins from envelope, stroma, and thylakoid sub-compartments Ferro et al., 2010. In addition to current knowledge regarding sub-plastidial localization, AT_CHLORO provides experimental data that allowed curated information regarding subcellular localizations of chloroplast proteins to be given. A specific focus was given to proteins that were identified in envelope fractions and for which expert functional annotation was provided. The present mini review shows the specificities of AT_CHLORO with respect to available information, data export options and recent improvements in data representation.
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