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Updated: May 18, 2026
10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Transcriptional elongation and alternative splicing
Gwendal Dujardin1, Celina Lafaille, Ezequiel Petrillo
1Departamento de Fisiología, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Biochimica Et Biophysica Acta
|September 15, 2012
Summary
Alternative splicing generates proteome diversity and is linked to transcription. Transcription elongation plays a key role in regulating alternative splicing, particularly through the kinetic model.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Alternative splicing is a major source of proteome diversity.
- Splicing is increasingly recognized as a cotranscriptional process.
- Understanding the interplay between transcription and splicing is crucial.
Purpose of the Study:
- To review the links between transcription and splicing.
- To emphasize the role of transcription elongation in alternative splicing regulation.
- To discuss the kinetic model of alternative splicing.
Main Methods:
- Literature review and synthesis.
- Discussion of existing models and findings.
- Focus on cotranscriptional processes.
Main Results:
- Crosstalk between transcription and splicing is significant.
- Transcription elongation kinetics influence alternative splicing outcomes.
- The kinetic model provides a framework for understanding this regulation.
Conclusions:
- Regulation of alternative splicing is intrinsically linked to transcription.
- Transcription elongation is a critical regulatory point for alternative splicing.
- Further research into cotranscriptional processes is warranted.
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There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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