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Updated: May 18, 2026
11:36
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
Published on: April 28, 2016
The study of ghrelin deacylation enzymes
Motoyasu Satou1, Hiroyuki Sugimoto
1Department of Biochemistry, Dokkyo Medical University School of Medicine, Mibu, Tochigi, Japan.
Methods in Enzymology
|September 15, 2012
Summary
Fatty acid modification of ghrelin is crucial for its function. This review explores the deacylation process and enzymes, revealing the physiological importance of deacylated ghrelin.
Area of Science:
- Biochemistry
- Endocrinology
- Molecular Biology
Background:
- Fatty acid modification of peptides, such as ghrelin, critically influences their functional properties.
- Ghrelin, a circulating hormone, regulates growth hormone release, appetite, and cellular functions.
- While acylated ghrelin binds the ghrelin receptor, deacylated ghrelin also exhibits distinct physiological activities.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying ghrelin processing, focusing on the deacylation process.
- To understand the physiological significance of deacylated ghrelin in vivo.
- To review methodologies for detecting and quantifying ghrelin and its deacylation activity.
Main Methods:
- Immunological and mass spectrometry-based detection of ghrelin.
- Quantification assays for ghrelin deacylase activity.
- Isolation and characterization of ghrelin deacylation enzymes from biological fluids and via heterologous expression.
Main Results:
- Established methods for ghrelin detection and activity quantification.
- Identified and characterized key enzymes responsible for ghrelin deacylation.
- Provided insights into the molecular basis of ghrelin processing.
Conclusions:
- Understanding ghrelin deacylation is vital for comprehending its full physiological role.
- Deacylated ghrelin possesses significant biological activities.
- The reviewed methodologies facilitate further research into ghrelin's complex functions.
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