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Related Experiment Videos

Physical mapping of mouse histone gene clusters.

B K Rasheed1, E C Whisenant, Y M Bhatnagar

  • 1Departments of Structural & Cellular Biology, University of South Alabama, College of Medicine, Mobile 36688.

Biochimica Et Biophysica Acta
|January 30, 1990
PubMed
Summary

Researchers isolated five mouse genomic clones using a chicken histone H2A probe. These clones, ranging from 11-16 kb, did not contain the complete set of histone genes, and their physical maps were constructed.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Histones are crucial proteins that package DNA into chromatin.
  • Understanding histone gene organization is vital for studying gene regulation and genome structure.

Purpose of the Study:

  • To isolate and characterize mouse genomic clones containing histone genes.
  • To investigate the organization and completeness of histone gene clusters in the mouse genome.

Main Methods:

  • Screening a mouse genomic library (lambda Charon 4A) using a chicken histone H2A probe.
  • Analyzing insert sizes of isolated clones (11-16 kb).
  • Constructing physical maps using partial- and double-restriction digests.

Main Results:

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  • Five distinct mouse genomic clones were successfully isolated.
  • None of the isolated clones contained the full complement of histone genes.
  • Physical maps were generated for each of the five clones.

Conclusions:

  • The isolated clones represent fragments of the mouse genome containing histone genes.
  • Further analysis is required to isolate clones encompassing the complete histone gene set.
  • Restriction mapping provides a foundational understanding of the genomic organization of these histone gene regions.