Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges and solutions in measuring commonly used biomarkers for drug-induced liver injury in a liver-on-a-chip platform.

Toxicology letters·2025
Same author

Toxicity of ubiquitous tire rubber antiozonant <i>N</i>-(1,3-dimethylbutyl)-<i>N</i>'-phenyl-<i>p</i>-phenylenediamine (6PPD) and its transformation product 6PPD-quinone (6PPD-Q) in primary human hepatocytes and liver spheroids.

Biochemistry and biophysics reports·2025
Same author

Assessing the developmental effects of fentanyl and impacts on lipidomic profiling using neural stem cell models.

Experimental biology and medicine (Maywood, N.J.)·2025
Same author

Biomarker-driven drug development for allergic diseases and asthma: An FDA public workshop.

The Journal of allergy and clinical immunology·2025
Same author

The incorporation of MALDI mass spectrometry imaging in studies to identify markers of toxicity following <i>in utero</i> opioid exposures in mouse fetuses.

Frontiers in toxicology·2024
Same author

Untargeted metabolomics and lipidomics in COVID-19 patient plasma reveals disease severity biomarkers.

Metabolomics : Official journal of the Metabolomic Society·2024

Related Experiment Video

Updated: May 18, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Metabolomics techniques in nanotoxicology studies.

Laura K Schnackenberg1, Jinchun Sun, Richard D Beger

  • 1Division of Systems Biology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA. Laura.Schnackenberg@fda.hhs.gov

Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2012
PubMed
Summary

Nanoparticle applications are growing, raising toxicity concerns. Metabolomics offers a high-throughput method to assess nanoparticle toxicity and understand mechanisms like oxidative stress using LC/MS and NMR.

More Related Videos

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Related Experiment Videos

Last Updated: May 18, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

  • Nanotechnology
  • Toxicology
  • Biochemistry

Background:

  • Nanoparticle development is rapidly expanding for applications in medicine and diagnostics.
  • Increased nanoparticle use necessitates robust methods for evaluating human toxicity.
  • Omics technologies, particularly metabolomics, are suitable for high-throughput toxicity screening.

Purpose of the Study:

  • To explore the application of metabolomics for assessing nanoparticle toxicity.
  • To investigate oxidative stress as a key mechanism in nanoparticle-induced toxicity.
  • To demonstrate the utility of LC/MS and NMR-based metabolomics in nanotoxicity studies.

Main Methods:

  • Utilizing liquid chromatography-mass spectrometry (LC/MS) for metabolomic analysis.
  • Employing nuclear magnetic resonance (NMR) spectroscopy for metabolomic profiling.
  • Applying metabolomics to screen for biomarkers of nanoparticle toxicity in vitro and in vivo.

Main Results:

  • Metabolomics can rapidly identify biomarkers associated with nanoparticle exposure.
  • Oxidative stress, a common toxicity pathway, can be effectively monitored via metabolomics.
  • LC/MS and NMR provide complementary data for mechanistic insights into nanotoxicity.

Conclusions:

  • Metabolomics is a powerful tool for high-throughput evaluation of nanoparticle toxicity.
  • This approach offers mechanistic understanding of nanotoxicity, including oxidative stress.
  • LC/MS and NMR-based metabolomics are valuable for safety assessments in nanotechnology.