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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Microbiota of Attine Ants' Gardens: Visualizing a Microbial Landscape by Scanning Electron Microscopy
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Applications of subsurface microscopy.

Laurene Tetard1, Ali Passian, Rubye H Farahi

  • 1Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2012
PubMed
Summary

A new technique called mode synthesizing atomic force microscopy (MSAFM) allows for label-free, subsurface imaging of nanoparticles within cells. This nondestructive method overcomes limitations of current imaging tools for biological and nanomaterial research.

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Area of Science:

  • Nanotechnology
  • Cell Biology
  • Microscopy

Background:

  • Characterizing nanomaterials within cells is crucial for understanding their biological effects.
  • Current imaging techniques often require labels or are limited to surface topography.
  • Subsurface nanoscale imaging within cells presents significant instrumentation challenges.

Purpose of the Study:

  • To develop a novel, label-free, and nondestructive method for subsurface nanoscale imaging within cells.
  • To visualize non-labeled nanoparticles embedded within cellular structures.
  • To overcome the limitations of existing high-resolution characterization tools.

Main Methods:

  • Utilizing atomic force microscopy (AFM) with a microcantilever probe.
  • Introducing mode synthesizing atomic force microscopy (MSAFM) by exciting the probe and sample at different frequencies.
  • Leveraging nonlinear tip-sample interactions to generate sum- and difference-frequency elastic waves.
  • Employing specific electronics to select and monitor synthesized imaging modes (amplitude and phase).

Main Results:

  • MSAFM successfully generated new imaging modes by mixing elastic waves.
  • The technique provided subsurface information, enabling visualization of internal structures.
  • Non-labeled nanoparticles embedded within cells were revealed through the acquired images.

Conclusions:

  • MSAFM offers a powerful new capability for nondestructive, label-free subsurface imaging at the nanoscale.
  • This technique can provide critical insights into nanomaterial-cell interactions.
  • MSAFM advances the field of cellular imaging and nanomaterial characterization within biological systems.