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Related Experiment Video

Updated: May 18, 2026

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology
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Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology

Published on: May 28, 2017

Methods for measuring tau pathology in transgenic mouse models.

Stefanie K Forest1, Christopher M Acker, Cristina d'Abramo

  • 1Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA.

Journal of Alzheimer'S Disease : JAD
|September 15, 2012
PubMed
Summary

This study compares methods for measuring insoluble tau in Alzheimer's disease (AD) and tauopathies. A new monoantibody ELISA offers a simple, quantitative approach for aggregated tau detection in human and mouse brains.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Tau pathology, including hyperphosphorylation and aggregation, is central to Alzheimer's disease (AD) and tauopathies.
  • Current methods for studying insoluble tau fractions (Sarkosyl, RIPA, insoluble) require large tissue samples and yield limited amounts.
  • Immunoblot densitometry provides only semiquantitative measurements of insoluble tau.

Purpose of the Study:

  • To compare the efficacy of three common methods for isolating insoluble tau fractions.
  • To evaluate the performance of immunoblot and ELISA analyses for quantifying tau species.
  • To introduce and validate a novel monoantibody ELISA for measuring aggregated tau.

Main Methods:

  • Comparison of Sarkosyl, RIPA, and insoluble preparation methods.

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  • Immunoblot and Enzyme-Linked Immunosorbent Assay (ELISA) analyses were employed.
  • Testing of a new monoantibody tau ELISA using various mouse models and human brain samples.

    • Main Results:

    • Significant correlations were observed between the three insoluble preparation methods for total tau and pS396/404 tau.
    • ELISA results showed significant correlation with immunoblot analyses.
    • The monoantibody assay correlated significantly with total tau ELISAs, indicating its utility for aggregated tau measurement.

    Conclusions:

    • All three insoluble preparation methods yield comparable results for measuring insoluble tau in mouse and human brains.
    • The novel monoantibody ELISA provides a straightforward and quantitative method for assessing aggregated tau levels.
    • This new assay simplifies the study of aggregated tau in neurodegenerative diseases.