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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Positron Emission Tomography

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Updated: May 18, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

MALDI in-source decay, from sequencing to imaging.

Delphine Debois1, Nicolas Smargiasso, Kevin Demeure

  • 1Mass Spectrometry Laboratory, GIGA-R, Department of Chemistry, University of Liège, Allée de la Chimie 3, 4000, Liège, Belgium.

Topics in Current Chemistry
|September 15, 2012
PubMed
Summary
This summary is machine-generated.

Matrix-assisted laser desorption/ionization (MALDI) with in-source decay (ISD) enables fragmentation of large biopolymers. This technique offers new perspectives for analyzing proteins, nucleic acids, and glycans, including tissue imaging.

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

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Last Updated: May 18, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

Area of Science:

  • Mass Spectrometry
  • Biochemistry
  • Analytical Chemistry

Background:

  • Matrix-assisted laser desorption/ionization (MALDI) is established for biopolymer identification and quantification.
  • MALDI typically produces intact singly charged ions, limiting tandem mass spectrometry (MS/MS) for large molecules (>3.5 kDa).
  • In-source decay (ISD) offers a prompt fragmentation method within the MALDI source.

Purpose of the Study:

  • To review the mechanisms and applications of in-source decay (ISD) in MALDI mass spectrometry.
  • To highlight advancements in MALDI-ISD for analyzing large biopolymers and complex biological samples.
  • To explore the potential of MALDI-ISD in top-down sequencing, PTM analysis, and tissue imaging.

Main Methods:

  • Review of existing literature on MALDI-ISD mechanisms and applications.
  • Discussion of thermal and radical-induced fragmentation pathways in the MALDI source.
  • Exploration of pseudo MS3 capabilities for intense fragments generated by ISD.

Main Results:

  • ISD is a mass-independent fragmentation technique applicable to large biopolymers.
  • New matrices and improved MALDI mass spectrometers enhance ISD yield and sensitivity.
  • MALDI-ISD facilitates top-down sequencing, PTM analysis, and MALDI-ISD tissue imaging.

Conclusions:

  • MALDI-ISD overcomes limitations of traditional MALDI MS/MS for large biomolecules.
  • Advancements in matrices, instrumentation, and software expand the utility of MALDI-ISD.
  • MALDI-ISD is a powerful tool for diverse applications in biopolymer analysis and biological imaging.