Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamines and cellular adenosine 3' :5'-cyclic monophosphate.

C Clô, C M Caldarera, B Tantini

    The Biochemical Journal
    |September 15, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Zinc can influence ornithine decarboxylase activity in rat thymus cells.

    Amino acids·2013
    Same author

    The role of extracellular conditions during CaCo-2 cells growth: a preliminary study for numerical model validation.

    European review for medical and pharmacological sciences·2011
    Same author

    Cardiac resynchronization therapy: could a numerical simulator be a useful tool in order to predict the response of the biventricular pacemaker synchronization?

    European review for medical and pharmacological sciences·2011
    Same author

    Difluoromethylornithine inhibits hypertrophic, pro-fibrotic and pro-apoptotic actions of aldosterone in cardiac cells.

    Amino acids·2009
    Same author

    Mesenchymal stem cell interaction with a non-woven hyaluronan-based scaffold suitable for tissue repair.

    Journal of anatomy·2008
    Same author

    Polyamine biosynthesis as a target to inhibit apoptosis of non-tumoral cells.

    Amino acids·2007

    Polyamines like spermine, spermidine, and putrescine decrease cyclic AMP levels in various cultured cells. This effect is linked to polyamine interactions with cellular phosphodiesterase activity.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Pharmacology

    Background:

    • Cellular cyclic AMP (cAMP) is a crucial second messenger regulating numerous physiological processes.
    • Polyamines are essential polycations involved in cell growth and differentiation.
    • The precise role of polyamines in modulating cAMP signaling pathways remains incompletely understood.

    Purpose of the Study:

    • To investigate the impact of different polyamines on intracellular cAMP concentrations across various cell types.
    • To explore whether polyamine-induced changes in cAMP are modulated by phosphodiesterase activity or hormonal stimulation.

    Main Methods:

    • Culturing diverse cell lines including chick-embryo heart cells, fibroblasts, neuroblastoma, glioma, and hybrid cells.
    • Treating cells with varying concentrations of spermine, spermidine, and putrescine.

    Related Experiment Videos

  • Measuring cellular cAMP levels with and without phosphodiesterase inhibitors or hormonal stimulation (noradrenaline, prostaglandin E1, adenosine, isoproterenol).

    • Main Results:

    • Low concentrations (1 microM) of spermine significantly decreased cAMP levels in all tested cell types.
    • Spermidine (10 microM) and putrescine (0.1 microM) also reduced cellular cAMP.
    • Higher polyamine concentrations showed minimal or slightly increased cAMP levels.
    • The cAMP reduction persisted in the presence of phosphodiesterase inhibitors or hormonal stimulation.

    Conclusions:

    • Polyamines, particularly at lower concentrations, exert a suppressive effect on cellular cAMP levels.
    • The observed decrease in cAMP is likely mediated by the influence of polyamines on cellular phosphodiesterase activity.
    • These findings suggest a novel regulatory mechanism for cAMP signaling involving polycationic molecules.