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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...

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Related Experiment Video

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Isolation and Transcriptome Analysis of Plant Cell Types
08:53

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Single-cell-type proteomics: toward a holistic understanding of plant function.

Shaojun Dai1, Sixue Chen

  • 1Department of Biology, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.

Molecular & Cellular Proteomics : MCP
|September 18, 2012
PubMed
Summary

Analyzing single plant cell proteins reveals specialized functions and organismal insights. This proteomics approach offers high resolution for novel discoveries and understanding plant biology.

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Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

Area of Science:

  • Plant biology
  • Proteomics
  • Cellular analysis

Background:

  • Multicellular plants possess diverse cell types with specialized functions.
  • Traditional plant proteomics often analyzes mixed cell populations, limiting single-cell insights.
  • Recent advances enable single-cell-type proteomics, offering higher resolution.

Purpose of the Study:

  • To review recent developments in plant single-cell-type proteomics.
  • To discuss the application of single-cell proteomics in understanding plant cell functions.
  • To explore technical challenges and future integration with other 'omics'.

Main Methods:

  • Focus on single-cell-type proteomics techniques.
  • Review of studies analyzing specific plant cell types (pollen, guard cells, mesophyll, etc.).
  • Discussion of integrating proteomics with transcriptomics and metabolomics.

Main Results:

  • Single-cell-type proteomics has demonstrated utility in identifying specific cell functions.
  • High-resolution proteomic analysis is expected to uncover novel functions within individual plant cells.
  • The approach holds potential for a holistic understanding of plant biology.

Conclusions:

  • Plant single-cell-type proteomics is a powerful approach for dissecting cellular functions.
  • Overcoming technical challenges is key to expanding its application across all plant cell types.
  • Integrating proteomics with transcriptomics and metabolomics will provide comprehensive insights into plant function.