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Related Experiment Videos

Ascorbic acid accumulation in plated human neutrophils.

P Washko1, D Rotrosen, M Levine

  • 1Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

FEBS Letters
|January 15, 1990
PubMed
Summary
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Human neutrophils actively absorb ascorbic acid (vitamin C) when it is present in the surrounding environment. This vitamin C uptake by neutrophils requires calcium and magnesium ions for optimal transport.

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Immunology

Background:

  • Neutrophils are critical immune cells involved in the inflammatory response.
  • Ascorbic acid (vitamin C) is a vital antioxidant with potential roles in immune function.
  • Understanding nutrient uptake mechanisms in immune cells is crucial for immune health research.

Purpose of the Study:

  • To investigate the uptake kinetics of ascorbic acid in isolated human neutrophils.
  • To determine the influence of extracellular ascorbic acid concentrations on neutrophil accumulation.
  • To identify factors, such as divalent cations, affecting ascorbic acid transport in neutrophils.

Main Methods:

  • Isolated human neutrophils were cultured and plated for experimental analysis.
  • High-performance liquid chromatography (HPLC) with coulometric electrochemical detection was employed to quantify ascorbic acid.

Related Experiment Videos

  • Scintillation spectrometry of radiolabeled [14C]ascorbic acid coupled with HPLC was utilized to measure transport dynamics.

    • Main Results:

    • Freshly isolated neutrophils contained an endogenous concentration of 1.3 mM ascorbic acid.
    • Neutrophils significantly accumulated greater amounts of ascorbic acid when physiologic concentrations were present extracellularly.
    • Ascorbic acid uptake was dependent on the presence of calcium (Ca2+) and magnesium (Mg2+) ions in the buffer.

    Conclusions:

    • Human neutrophils possess an active mechanism for ascorbic acid uptake.
    • The transport of ascorbic acid into neutrophils is modulated by extracellular vitamin C levels and requires specific divalent cations.
    • The described HPLC and scintillation spectrometry methods are suitable for studying ascorbic acid transport in neutrophils.