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Related Experiment Video

Updated: May 18, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Multifunctional sample preparation kit and on-chip quantitative nucleic acid sequence-based amplification tests for

Xinyan Zhao¹, Tao Dong

¹(IMST) Department of Micro and Nano Systems Technology, Faculty of Engineering and Marine Sciences, (HiVE) Vestfold University College, Norway.

Analytical Chemistry

|September 19, 2012

Summary

This summary is machine-generated.

A new microfluidic platform enables efficient, low-cost collection and quantitative detection of aquatic microorganisms. This system simplifies environmental monitoring of microbial communities using quantitative nucleic acid sequence-based amplification (Q-NASBA).

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Last Updated: May 18, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Environmental microbiology
Microfluidics
Molecular diagnostics

Background:

Aquatic environments harbor diverse microbial communities crucial for ecosystem health.
Accurate and efficient monitoring of aquatic microorganisms is essential for environmental assessment.
Current methods for microbial analysis can be complex, costly, and time-consuming.

Purpose of the Study:

To develop a novel, integrated microfluidic platform for the quantitative analysis of aquatic microorganisms.
To enable low-cost, efficient, and simultaneous detection of microbial communities in environmental samples.
To streamline the process from sample collection to quantitative nucleic acid sequence-based amplification (Q-NASBA) detection.

Main Methods:

A microfluidic platform integrating a membrane-based sampling module, a sample preparation cassette, and a 24-channel Q-NASBA chip.
Evaluation of commercial membrane filters using model microorganisms (Saccharomyces cerevisiae, Escherichia coli, Staphylococcus aureus).
Development of a novel analytical algorithmic method for on-chip Q-NASBA assays.

Main Results:

The platform demonstrated efficient collection and concentration of microbial communities from aquatic samples.
A sample preparation cassette was designed for direct DNA/RNA extraction from captured cells.
The system achieved quantitative and simultaneous detection of multiple microorganisms using Q-NASBA chips compatible with standard microplate readers.

Conclusions:

The developed multifunctional microfluidic system offers a promising solution for quantitative environmental investigations of aquatic microorganisms.
The integrated platform simplifies sample handling, preparation, and analysis, reducing costs and increasing efficiency.
Further standardization and simplification are recommended for broader application in ecological studies.