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Updated: May 18, 2026

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
Published on: June 23, 2023
Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells
Venkatramreddy Velma1, Zunamys I Carrero, Carmen B Allen
1Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
Abstract:
Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.
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