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Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

Tripeptidyl peptidase II in human oral squamous cell carcinoma

Katsuya Usukura1, Atsushi Kasamatsu, Atsushi Okamoto

  • 1Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

Journal of Cancer Research and Clinical Oncology
|September 19, 2012
PubMed
Abstract

Insights

Overexpression of tripeptidyl peptidase II (TPP2) is frequent in oral squamous cell carcinoma (OSCC) and linked to tumor progression. TPP2 may drive OSCC development through spindle assembly checkpoint activation.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Tripeptidyl peptidase II (TPP2) is a serine peptidase involved in DNA repair, cell division, and apoptosis.
  • Understanding TPP2's role in oral squamous cell carcinoma (OSCC) is crucial for targeted therapies.

Purpose of the Study:

  • To investigate TPP2 expression levels in OSCC.
  • To elucidate the functional mechanisms of TPP2 in OSCC progression.

Main Methods:

  • Quantitative reverse transcriptase-polymerase chain reaction and immunoblotting were used to analyze TPP2 expression in OSCC cell lines.
  • Cellular proliferation and spindle assembly checkpoint (SAC) molecules (MAD2, CCNB1) were assessed following TPP2 knockdown.
  • Immunohistochemistry (IHC) evaluated TPP2 expression in 108 primary OSCC specimens and correlated it with clinicopathologic features.

Main Results:

  • TPP2 mRNA and protein were significantly upregulated in OSCC cells compared to normal oral keratinocytes.
  • TPP2 suppression inhibited cellular proliferation and affected MAD2 localization and CCNB1 expression in OSCC cells.
  • Elevated TPP2 expression in primary OSCCs correlated significantly with larger tumor size.

Conclusions:

  • Overexpression of TPP2 is a common event in oral carcinogenesis.
  • TPP2 may contribute to OSCC progression by activating the spindle assembly checkpoint (SAC).

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