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Determination of the packaging capacity of bacteriophage VWB
J Anné1, L Van Mellaert, H Eyssen
1Department of Microbiology, Rega Institute, Katholieke Universiteit Leuven, Belgium.
Journal of Bacteriology
|February 1, 1990
Abstract:
VWB is a temperate bacteriophage whose chromosome has cohesive ends. VWB can stably package modified chromosomes that contain insertions of up to about 4 kilobases of foreign DNA. Phage particles containing extra DNA differ from the wild type in their increased sensitivity to chelating agents. Because of these properties, VWB is a promising cloning vector for Streptomyces venezuelae.