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Related Experiment Video

Updated: May 18, 2026

Procedure for Fabricating Biofunctional Nanofibers
09:39

Procedure for Fabricating Biofunctional Nanofibers

Published on: September 10, 2012

Procedure for fabricating biofunctional nanofibers.

Jereme Doss1, Omotunde Olubi, Biswajit Sannigrahi

  • 1Department of Chemistry, Clark Atlanta University, Atlanta, GA, USA.

Journal of Visualized Experiments : Jove
|September 19, 2012
PubMed
Summary
This summary is machine-generated.

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Researchers developed functional nanofibers for biosensing. These electrospun nanofibers, decorated with 2,4-dinitrophenyl (DNP) groups, specifically bind to anti-DNP Immunoglobulin E (IgE), showing potential for biomarker detection devices.

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Electrospinning is a versatile technique for creating functional nanofibers.
  • Functionalized materials are crucial for developing advanced biosensors.
  • Specific molecular recognition is key for biomarker detection.

Purpose of the Study:

  • To synthesize functional polymers for specific protein binding.
  • To fabricate electroactive nanofibers for biosensing applications.
  • To investigate the interaction between functionalized nanofibers and a model protein.

Main Methods:

  • Anionic living polymerization to synthesize a functional triblock copolymer: α,ω-bi[2,4-dinitrophenyl caproic][poly(ethylene oxide)-b-poly(2-methoxystyrene)-b-poly(ethylene oxide)] (CDNP-PEO-P2MS-PEO-CDNP).

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Last Updated: May 18, 2026

Procedure for Fabricating Biofunctional Nanofibers
09:39

Procedure for Fabricating Biofunctional Nanofibers

Published on: September 10, 2012

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
07:38

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation
13:02

Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation

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  • DCC coupling to attach N-2,4-DNP-∈-amino caproic acid to the polymer.
  • Electrospinning of functional polymers with single-wall carbon nanotubes (SWCNTs) to create nanofibers.
  • Fluorescence spectroscopy to confirm protein-nanofiber interactions.

    • Main Results:

    • Successfully synthesized and characterized the functional polymer CDNP-PEO-P2MS-PEO-CDNP.
    • Prepared electroactive nanofibers (100-500 nm diameter) via electrospinning.
    • Demonstrated specific binding of anti-DNP IgE to the DNP functional groups on the nanofibers.
    • Observed rapid binding equilibrium with solution IgE using a water-soluble complex.

    Conclusions:

    • Functionalized nanofibers show promise as components of biomarker detection devices.
    • The developed material enables specific molecular recognition for biosensing.
    • Electrospun nanofibers offer a platform for sensitive and specific biomaterial-protein interaction studies.