Related Experiment Video
Updated: May 18, 2026
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Tuf and secY PCR amplification and genotyping of phytoplasmas
Xavier Foissac1, Jean-Luc Danet, Sylvie Malembic-Maher
1UMR1332 Fruit Biology and Pathology, INRA, University of Bordeaux, Villenave d'Ornon, France.
Abstract:
Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence.
