Jove
Visualize
Contact Us

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OT-Mation: an open-source code for parsing CSV files into Python scripts for control of OT-2 liquid-handling robotics.

Synthetic biology (Oxford, England)·2025
Same author

Plant pest surveillance: from satellites to molecules.

Emerging topics in life sciences·2021
Same author

A pond-side test for Guinea worm: Development of a loop-mediated isothermal amplification (LAMP) assay for detection of Dracunculus medinensis.

Experimental parasitology·2020
Same author

Advances in molecular phytodiagnostics - new solutions for old problems.

European journal of plant pathology·2020
Same author

Development of a reverse transcription-loop- mediated isothermal amplification (LAMP) assay for the rapid detection of onion yellow dwarf virus.

Journal of virological methods·2019
Same author

Development of Loop-Mediated Isothermal Amplification Assays for the Detection of Seedborne Fungal Pathogens Fusarium fujikuroi and Magnaporthe oryzae in Rice Seed.

Plant disease·2019
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 18, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

In-field diagnostics using loop-mediated isothermal amplification.

Jenny Tomlinson1

  • 1The Food and Environment Research Agency, Sand Hutton, York, UK. jenny.tomlinson@fera.gsi.gov.uk

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2012
PubMed
Summary

Loop-mediated isothermal amplification (LAMP) offers rapid, sensitive, and specific detection of target organisms without thermal cycling. This method is ideal for field deployment, providing laboratory-level accuracy in simpler settings.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

Related Experiment Videos

Last Updated: May 18, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

Area of Science:

  • Molecular biology
  • Biotechnology
  • Plant pathology

Background:

  • Polymerase chain reaction (PCR) requires thermal cycling, limiting its field applicability.
  • Phytoplasmas are plant pathogens that are difficult to detect using conventional methods.
  • There is a need for rapid, sensitive, and specific diagnostic tools for phytoplasma detection, especially in field settings.

Purpose of the Study:

  • To highlight the advantages of Loop-mediated isothermal amplification (LAMP) for detecting target organisms.
  • To demonstrate the suitability of LAMP for the rapid, field-deployable detection of phytoplasmas.
  • To compare the sensitivity and specificity of LAMP with traditional laboratory methods.

Main Methods:

  • Loop-mediated isothermal amplification (LAMP) was employed for nucleic acid amplification.
  • Assays were developed for laboratory use and adapted for field deployment.
  • Sensitivity and specificity were evaluated against established laboratory diagnostic techniques.

Main Results:

  • LAMP does not require thermal cycling, simplifying the amplification process.
  • LAMP assays demonstrated high sensitivity and specificity for phytoplasma detection.
  • The method proved suitable for rapid detection in field conditions.

Conclusions:

  • LAMP is a valuable tool for the rapid and accurate detection of phytoplasmas.
  • The simplicity of LAMP makes it ideal for field-based diagnostics.
  • LAMP offers a viable alternative to complex and time-consuming laboratory methods for phytoplasma identification.