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Related Experiment Video

Updated: May 18, 2026

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

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Published on: July 13, 2016

RGD-Dependent Epithelial Cell-Matrix Interactions in the Human Intestinal Crypt.

Yannick D Benoit¹, Jean-François Groulx, David Gagné

¹Département d'Anatomie et Biologie Cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4 ; Pharmacology Department, Weill Cornell Medical College, New York, NY 10065, USA.

Journal of Signal Transduction

|September 19, 2012

Summary

Extracellular matrix (ECM) and integrin interactions are vital for cell structure and function. This review focuses on RGD-dependent integrins in human intestinal epithelial cells, highlighting their role in maintaining homeostasis.

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Area of Science:

Cell Biology
Biochemistry
Physiology

Background:

Extracellular matrix (ECM) interactions with cell surface receptors, particularly integrins, establish critical connections between the cellular microenvironment and the cytoskeleton.
These connections are fundamental for maintaining cell adhesion structure integrity and transducing intracellular signals that regulate cell organization and function.
Epithelial homeostasis relies on specific ECM receptor subgroups, including RGD peptide-dependent integrins.

Purpose of the Study:

To review recent findings on the interactions between RGD-dependent integrins and the extracellular matrix.
To elucidate the roles of these interactions in the context of human intestinal epithelial crypt cells.

Main Methods:

Literature review of recent research on ECM-integrin interactions.
Focus on studies specifically investigating RGD peptide-dependent integrins.
Analysis of findings related to human intestinal epithelial crypt cell biology.

Main Results:

RGD-dependent integrins are crucial mediators of ECM-cell binding in intestinal epithelium.
These interactions influence intracellular signaling pathways regulating epithelial cell organization.
Dysregulation of these interactions can impact epithelial homeostasis.

Conclusions:

RGD-dependent ECM-integrin interactions are essential for maintaining human intestinal epithelial homeostasis.
Understanding these mechanisms provides insights into epithelial cell function and potential therapeutic targets.