Related Experiment Video
Updated: May 6, 2026
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
Identification of markers associated with highly aggressive metastatic phenotypes using quantitative comparative
Mikkel G Terp1, Rikke R Lund, Ole N Jensen
1Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Researchers identified key proteins linked to aggressive breast cancer metastasis. Leucine-rich repeat containing 59 (LRRC59) correlated with aggressiveness, while CD59 and chondroitin sulfate proteoglycan 4 (CSPG4) showed inverse correlation.
Area of Science:
- Oncology
- Proteomics
- Cancer Biology
Background:
- Metastasis, the spread of cancer, is a complex process poorly understood.
- Aggressive tumor cells drive rapid development of distant metastases.
- Identifying proteins associated with aggressive phenotypes is crucial for understanding metastasis.
Purpose of the Study:
- To identify proteins associated with cancer cell aggressiveness.
- To investigate the role of specific proteins in breast cancer metastasis.
Main Methods:
- Comparative, quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteome analysis.
- Utilized three isogenic human breast cancer cell lines with varying metastatic potentials.
- Confirmed altered protein expression using immunocytochemistry and immunohistochemistry.
Main Results:
- Identified 414 proteins, with 44 showing altered expression between metastatic and non-metastatic cell lines.
- Leucine-rich repeat containing 59 (LRRC59) correlated with metastasis aggressiveness.
- CD59 and chondroitin sulfate proteoglycan 4 (CSPG4) showed an inverse correlation with metastatic capability.
- Confirmed these proteins are associated with metastasis aggressiveness, not colonization.
Conclusions:
- Provided novel insights into key proteins linked to breast cancer metastatic potential.
- Identified LRRC59, CD59, and CSPG4 as candidates for further research in breast cancer metastasis.
More Related Videos
10:37Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
09:00A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
Published on: April 18, 2025