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Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
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Transfection of Plasmodium falciparum
Melanie Rug1, Alexander G Maier
1Research School of Biology, The Australian National University, Canberra, ACT, Australia. rug@wehi.edu.au
Methods in Molecular Biology (Clifton, N.J.)
|September 20, 2012
Abstract:
Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.
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