Related Experiment Video
Updated: May 18, 2026
Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages
Published on: May 30, 2019
Transfection of rodent malaria parasites
Nisha Philip1, Rachael Orr, Andrew P Waters
1Division of Infection and Immunity Glasgow Biomedical Research Centre, Faculty of Biomedical Life Sciences and Wellcome Trust Center for Molecular Parasitology, University of Glasgow, Glasgow, UK.
Abstract:
Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.
