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Updated: May 18, 2026

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
Published on: November 22, 2024
Experimental tools for the study of protein phosphorylation in Plasmodium
Dominique Dorin-Semblat1, Andrew R Bottrill, Lev Solyakov
1Centre National de Transfusion Sanguine, Université Paris, PARIS CEDEX, France.
Abstract:
The central role played by protein phosphorylation in the regulation of eukaryotic cellular processes calls for detailed investigations of this phenomenon in malaria parasites. Here, we describe protocols to measure the activity of protein kinases (using either recombinant proteins or native enzymes purified from parasite extracts), and outline procedures to identify phosphorylation sites on parasite proteins following a mass spectrometry approach.
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