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[Saline solution as culture media from a viewpoint of nosocomial bacteremia]
Verónica Vanesa Infante1, Ana María Cano, Humberto Medina Valdovinos
1Universidad de Guanajuato y Hospital Regional de Alta Especialidad del Bajío.
Introduction:
Nutrient-containing aqueous solutions for parenteral use are susceptible of microbial contamination, beeing an important cause of serious infectious complications. Objective. To determine the capacity of organisms for growing in saline as compared with dextrose solution and tri-destilled sterile water.
Material And Methods:
Experimental controlled study. Different microbial strains were innoculated in aqueous solutions (5% dextrose and 0.9% saline) as well as tri-destilled sterile water. Results were analized using the ANOVA test for repeated measurements.
Results:
In 0.9% saline solution, all the Enterobacteriaceae strains tested had a significant increase in their bacterial quantification in the analized time period (F = 8.35, p = 0.0145). For most organisms, the growth was even better than the one observed in nutrient-containing solution.
Conclusions:
The 0.9% saline solution can support significative growing of potentially pathogenic bacteria. We recommend a strict compliance to the good nursing standards when handling this kind of solutions.
Insights
0.9% saline solution supports significant bacterial growth, even exceeding that in nutrient solutions. This highlights the risk of microbial contamination in parenteral fluids and the need for strict aseptic techniques.
Area of Science:
- Microbiology
- Infectious Diseases
- Parenteral Nutrition
Context:
- Parenteral solutions are vulnerable to microbial contamination, leading to severe infections.
- Understanding microbial growth in common intravenous fluids is crucial for patient safety.
Purpose:
- To compare the microbial growth capacity of 0.9% saline solution against dextrose solution and sterile water.
- To identify risks associated with microbial contamination in parenteral fluids.
Summary:
- Enterobacteriaceae strains showed significant growth in 0.9% saline solution over time.
- Bacterial quantification increased significantly in saline, often surpassing growth in nutrient-containing solutions.
- ANOVA test confirmed statistically significant bacterial proliferation in saline.
Impact:
- 0.9% saline solution can support substantial growth of potentially pathogenic bacteria.
- Recommends strict adherence to good nursing practices to prevent contamination.
- Emphasizes the critical need for aseptic techniques in handling parenteral solutions.
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