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Direct-write patterning of bacterial cells by dip-pen nanolithography.

Jieun Kim¹, Young-Hun Shin, Seong-Hun Yun

¹Department of Polymer Science and Technology, Korea National University of Transportation, Chungju 380-702, Korea.

Journal of the American Chemical Society

|September 21, 2012

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This study introduces a novel "stamp-on" dip-pen nanolithography (DPN) method for precisely patterning live bacterial cells. The technique overcomes previous size limitations, enabling the creation of single-cell or multi-layer bacterial arrays.

Area of Science:

Biotechnology
Nanotechnology
Microbiology

Background:

Dip-pen nanolithography (DPN) excels at creating nano/microarrays of various materials.
Transporting large materials like bacteria with DPN is challenging due to water meniscus size limitations.

Purpose of the Study:

To develop a straightforward "stamp-on" DPN method for patterning micrometer-sized bacterial cells.
To overcome the size limitations of traditional DPN for biological material transport.

Main Methods:

Utilized a nanostructured poly(2-methyl-2-oxazoline) hydrogel-coated tip with carrier agents.
Employed a "stamp-on" DPN approach for direct-write patterning of Escherichia coli JM 109 cells.
Modulated ink solution viscosity to control deposition of single cells or multiple layers.

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Main Results:

Successfully generated patterns of micrometer-sized Escherichia coli JM 109 bacterial cells.
Demonstrated deposition of single bacterial cell arrays and multi-layer arrays.
Confirmed bacterial viability post-DPN patterning on Luria-Bertani-agar surfaces via fluorescence microscopy.

Conclusions:

The novel "stamp-on" DPN method effectively overcomes size limitations for patterning live bacterial cells.
This technique offers precise control over bacterial cell array generation, from single cells to multi-layer structures.
The method preserves bacterial viability, opening possibilities for advanced bio-patterning applications.