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Compact Quantum Dots for Single-molecule Imaging
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Published on: October 9, 2012

Colloidal quantum dots as saturable fluorophores.

Osip Schwartz1, Ron Tenne, Jonathan M Levitt

  • 1Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel 76100. osip.schwarz@weizmann.ac.il

ACS Nano
|September 21, 2012
PubMed
Summary

Colloidal quantum dots (QDs) show improved photostability under pulsed light at low repetition rates. This finding enhances the potential of QDs for advanced fluorescence microscopy and spectroscopy applications.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Physical Chemistry

Background:

  • Colloidal quantum dots (QDs) are widely used in optical applications.
  • However, intense illumination causes intermittent fluorescence and photobleaching in QDs.
  • Understanding QD photostability is crucial for their effective utilization.

Purpose of the Study:

  • To investigate the photostability of common colloidal quantum dots (QDs) under pulsed excitation.
  • To determine the effect of excitation power and repetition rate on QD fluorescence and photobleaching.
  • To explore mechanisms underlying QD blinking and photodamage.

Main Methods:

  • Studied fluorescence of two types of colloidal quantum dots (QDs).
  • Utilized pulsed laser excitation with varied power and repetition rates.
  • Analyzed QD emission intermittency and photobleaching under different illumination conditions.

Main Results:

  • QD photostability significantly improved at low repetition rates.
  • Prolonged optical saturation of QDs was achieved without apparent photodamage.
  • QD blinking is likely facilitated by light absorption in a transient state with microsecond decay.

Conclusions:

  • Low repetition rates enhance the photostability of colloidal quantum dots (QDs) under intense illumination.
  • This improved photostability opens new avenues for fluorescence microscopy and spectroscopy.
  • QD blinking dynamics are linked to transient excited states.