Cascade imaging of proteolytic pathways in cancer cells using fluorescent protein-conjugated gold nanoquenchers

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Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
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Published on: November 14, 2025

Kyoungsook Park1, Jinyoung Jeong, Bong Hyun Chung

  • 1BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Major of Nanobiotechnology, University of Science and Technology, Gwahangno, Yuseong, Daejeon 305-806, Republic of Korea.

Chemical Communications (Cambridge, England)
|September 21, 2012
PubMed

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...