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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
16:19

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Published on: September 1, 2009

Sperm aneuploidy testing using fluorescence in situ hybridization.

Benjamin R Emery1

  • 1Andrology and IVF Laboratories, Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA. bemery@hsc.utah.edu

Methods in Molecular Biology (Clifton, N.J.)
|September 21, 2012
PubMed
Summary
This summary is machine-generated.

Sperm aneuploidy screening, using fluorescent in situ hybridization (FISH), aids in diagnosing male infertility. This method analyzes sperm chromosome abnormalities to guide treatment decisions for affected men.

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Area of Science:

  • Reproductive biology
  • Human genetics
  • Medical diagnostics

Background:

  • Sperm aneuploidy screening is crucial for diagnosing male factor infertility.
  • Human sperm karyotyping, developed in the 1970s, identified at-risk populations like those with teratozoospermia, oligozoospermia, or translocations.
  • Current methods utilize interphase chromosome analysis.

Purpose of the Study:

  • To detail the protocol for sperm aneuploidy screening using fluorescent in situ hybridization (FISH).
  • To establish standardized procedures for specimen preparation, FISH probe hybridization, and scoring criteria.
  • To enhance the diagnostic accuracy of sperm ploidy analysis.

Main Methods:

  • Application of fluorescent in situ hybridization (FISH) on interphase sperm nuclei.
  • Enumeration of labeled chromosomes to determine sperm ploidy.
  • Evaluation of five to seven chromosomes per ejaculate to quantify aneuploid sperm percentage.

Main Results:

  • Established a detailed protocol for sperm aneuploidy screening.
  • Defined criteria for specimen preparation, FISH hybridization, destaining, and scoring.
  • Enabled accurate determination of aneuploid sperm percentages in male infertility patients.

Conclusions:

  • Sperm aneuploidy screening via FISH is a valuable diagnostic tool for male infertility.
  • The detailed protocol facilitates consistent and reliable assessment of sperm chromosome abnormalities.
  • This method aids in determining appropriate treatment strategies for male factor infertility.