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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 18, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Flow cytometric methods for sperm assessment.

Vanesa Robles1, Felipe Martínez-Pastor

  • 1INDEGSAL, University of León, León, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|September 21, 2012
PubMed
Summary
This summary is machine-generated.

Flow cytometry offers versatile sperm analysis. This study details three methods using SYBR-14/propidium iodide for viability, PNA-FITC/PI for acrosomal integrity, and JC-1 for mitochondrial potential, all analyzable with basic flow cytometers.

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Last Updated: May 18, 2026

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Published on: April 21, 2022

Fluorimetric Techniques for the Assessment of Sperm Membranes
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Published on: November 28, 2018

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
05:44

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

Area of Science:

  • Reproductive Biology
  • Cellular Biology
  • Biotechnology

Background:

  • Flow cytometry is a powerful tool for analyzing multiple sperm parameters.
  • Assessing sperm viability, acrosomal integrity, and mitochondrial potential are crucial for reproductive health evaluations.
  • Standardized flow cytometry protocols enhance the reliability of sperm quality assessments.

Purpose of the Study:

  • To present three distinct flow cytometry-based methods for evaluating key sperm quality parameters.
  • To demonstrate the utility of specific fluorescent stains for detailed sperm analysis.
  • To highlight the applicability of these methods using standard flow cytometry equipment.

Main Methods:

  • Sperm viability assessment using the double stain SYBR-14 and propidium iodide (PI).
  • Acrosomal integrity and viability assessment using PNA-FITC and PI double staining.
  • Mitochondrial membrane potential evaluation utilizing the JC-1 stain.

Main Results:

  • The described methods allow for the simultaneous assessment of multiple sperm characteristics.
  • SYBR-14/PI effectively differentiates viable from non-viable sperm cells.
  • PNA-FITC/PI provides insights into acrosomal status alongside viability, while JC-1 quantifies mitochondrial function.

Conclusions:

  • These three flow cytometry protocols provide a comprehensive approach to sperm quality assessment.
  • The methods are adaptable for use with basic flow cytometry instrumentation, making them widely accessible.
  • Accurate evaluation of sperm viability, acrosomal integrity, and mitochondrial potential is achievable through these techniques.