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Related Concept Videos

Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Transgenic Organisms00:53

Transgenic Organisms

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Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
06:03

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Published on: November 1, 2018

Methods for sperm-mediated gene transfer.

Marialuisa Lavitrano1, Roberto Giovannoni, Maria Grazia Cerrito

  • 1Department of Surgical Sciences, University of Milano-Bicocca, Monza, Italy. marialuisa.lavitrano@unimib.it

Methods in Molecular Biology (Clifton, N.J.)
|September 21, 2012
PubMed
Summary
This summary is machine-generated.

Sperm-mediated gene transfer is an efficient biotechnological tool for creating genetically modified animals. This method utilizes sperm

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Area of Science:

  • Biotechnology
  • Genetics
  • Animal Science

Background:

  • Transgenic technologies are crucial for generating genetically modified animals.
  • These animals have applications in research, medicine, veterinary science, and agriculture.
  • Existing transgenic methods can be complex and require optimization.

Purpose of the Study:

  • To describe sperm-mediated gene transfer as a potent transgenic technology.
  • To highlight the efficiency of this method in producing genetically modified animals.

Main Methods:

  • Sperm cells' natural ability to bind and internalize exogenous DNA is exploited.
  • Sperm cells carry the internalized DNA to the oocyte during fertilization.
  • This process results in the efficient generation of genetically modified offspring.

Main Results:

  • Sperm-mediated gene transfer achieves high efficiency in producing genetically modified animals.
  • The method leverages the inherent biological functions of sperm.

Conclusions:

  • Sperm-mediated gene transfer is a highly efficient and valuable technique within transgenic technologies.
  • This method offers a practical approach for generating genetically modified animals for diverse applications.