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Updated: May 18, 2026

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System
08:15

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Published on: April 11, 2025

Knockdown of receptor-interacting serine/threonine protein kinase-2 (RIPK2) affects EMT-associated gene expression in

Shuang Wu1, Tatsuo Kanda, Shingo Nakamoto

  • 1Department of Medicine and Clinical Oncology, Chiba University, Graduate School of Medicine, Chuo-ku, Chiba, Japan.

Anticancer Research
|September 21, 2012
PubMed
Abstract

Insights

Receptor-interacting serine/threonine protein kinase-2 (RIPK2) knockdown reduced epithelial-to-mesenchymal transition (EMT) gene expression in liver cancer cells. This suggests RIPK2 influences hepatic cell migration and could impact liver carcinogenesis and regeneration.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • Receptor-interacting serine/threonine protein kinase-2 (RIPK2) is implicated in tumor progression, differentiation, and wound repair.
  • Investigating RIPK2's role in hepatocellular carcinoma (HCC) is crucial for understanding liver cancer development.

Purpose of the Study:

  • To determine the effect of RIPK2 knockdown on epithelial-to-mesenchymal transition (EMT)-associated gene expression in human hepatoma cells.
  • To elucidate RIPK2's potential role in hepatic cell migration.

Main Methods:

  • Generated HepG2 cells with stable RIPK2 knockdown using shRNA (HepG2-shRIPK2).
  • Utilized PCR-based arrays to compare the expression of 84 EMT-associated genes between HepG2-shRIPK2 and control cells.

Main Results:

  • RIPK2 knockdown significantly down-regulated mRNA expression of key EMT markers including JAG1, PAI1, CDH1, SNAI2, KRT19, VIM, and SIP1.
  • Specifically, nuclear factor kappa B (NF-κB)-dependent PAI1 and VIM gene expressions were reduced upon RIPK2 knockdown.

Conclusions:

  • RIPK2 plays a role in regulating EMT-associated gene expression in hepatoma cells.
  • RIPK2 knockdown affects hepatic cell migration, suggesting its involvement in liver carcinogenesis and regeneration processes.

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