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Updated: May 18, 2026

A Method to Study de novo Formation of Chromatin Domains
Published on: August 23, 2019
A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
Bettina M Lengsfeld1, Kayla N Berry, Sharmistha Ghosh
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Abstract:
Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.
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