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[Effects of jin chai antiviral capsule on IFITM3 expression in mice]
Ju-Ying Zhong1, Xiao-Lan Cui, Yu-Jing Shi
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Abstract:
This study is to investigate the treatment of Jin Chai antiviral capsule for influenza virus FM1/47 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, real-time PCR and Western blot technique were used to detect the virus load and the interferoninducible transmembrane protein3 (IFITM3) in lung of mice at the 1st day, 3rd day, 5th day and 7th day after affected. The results showed that Jin Chai antiviral capsule in large, middle, small dose groups can decrease virus load significantly at each time point, after being affected (P<0.05, P<0.01), Jin Chai antiviral capsule can increase the interferoninducible transmembrane protein3 in lung of mice, large dose groups are significantly higher in expression of IFITM3 compared with model group at each time point (P<0.05, P<0.01). Middle dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day and the 5th day (P<0.05), small dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day (P<0.05). It can be concluded that Jin Chai antiviral capsule exerts antiviral effects against influenzavirus by raised expression of IFITM3.
Insights
Jin Chai antiviral capsules effectively reduced influenza virus load in mice. The treatment also boosted interferon-inducible transmembrane protein 3 (IFITM3) expression, demonstrating its antiviral properties.
Area of Science:
- Virology
- Pharmacology
- Immunology
Context:
- Influenza virus infections pose a significant global health challenge, necessitating the development of effective antiviral therapies.
- Interferon-inducible transmembrane protein 3 (IFITM3) plays a crucial role in cellular defense against viral entry and replication.
Purpose:
- To evaluate the efficacy of Jin Chai antiviral capsule in treating influenza virus (FM1/47, H1N1) infection in a mouse model.
- To investigate the impact of Jin Chai antiviral capsule on viral load and IFITM3 expression in mouse lungs.
Summary:
- Jin Chai antiviral capsule administration significantly reduced influenza virus load in mice across various dosages and time points (p<0.05, p<0.01).
- The treatment notably increased the expression of IFITM3 in mouse lungs, with higher doses showing significant upregulation compared to the control group (p<0.05, p<0.01).
- Specific dose-dependent increases in IFITM3 expression were observed at days 3 and 5 post-infection.
Impact:
- Jin Chai antiviral capsule demonstrates potential as a therapeutic agent for influenza infections.
- The findings suggest that Jin Chai antiviral capsule exerts its antiviral effects by enhancing the expression of IFITM3, a key antiviral host factor.
