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Related Experiment Video

Updated: May 18, 2026

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

Published on: August 31, 2018

Evolutionary insights into scleractinian corals using comparative genomic hybridizations.

Manuel Aranda¹, Michael K DeSalvo, Till Bayer

¹Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

|September 22, 2012

Summary

Coral gene expression can be studied across species using existing microarrays. Mitochondrial genes in robust corals show faster evolution than nuclear genes, impacting phylogenetic studies.

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Gas Chromatography-Mass Spectrometry-Based Targeted Metabolomics of Hard Coral Samples

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Published on: October 13, 2023

Area of Science:

Marine Biology
Genomics
Evolutionary Biology

Background:

Coral reefs are vital ecosystems facing global decline due to climate change and pollution.
Understanding coral molecular responses to stress is crucial for predicting population viability.
Microarray technology offers insights into coral stress responses.

Purpose of the Study:

To assess the utility of an Acropora palmata microarray for diverse coral species.
To identify rapidly evolving genes in corals using comparative genomic hybridizations.
To evaluate existing microarray platforms for heterologous gene expression studies.

Main Methods:

Comparative genomic hybridizations (CGH) were performed.
An Acropora palmata microarray with 13,546 cDNA clones was used.
Hybridizations were conducted with various coral species.

Main Results:

The A. palmata microarray is suitable for studying a broad range of coral species.
A higher proportion of unannotated genes were highly diverged, suggesting rapid evolution.
Mitochondrial genes in M. faveolata showed higher divergence than nuclear genes compared to A. palmata.

Conclusions:

Existing microarray platforms facilitate transcriptional analyses across coral species, enhancing understanding of stress responses and evolutionary divergence.
cDNA arrays can identify genomic divergence patterns.
Mitochondrion-encoded genes appear to evolve faster than nuclear genes in robust corals, a factor to consider for phylogenetic analyses.