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Related Experiment Video

Updated: May 18, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling.

Pamela N Nge1, Jayson V Pagaduan, Ming Yu

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.

Journal of Chromatography. A
|September 22, 2012
PubMed
Summary

This study presents a novel microfluidic technique for automated sample preparation, combining on-chip enrichment, fluorescence labeling, and purification for enhanced analysis. The method enables fast, miniaturized, and quantitative detection of biomolecules like proteins.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Microfluidics

Background:

  • Microfluidic devices are crucial for developing micro total analysis systems.
  • Automated sample preparation is essential for efficient microfluidic analyses.

Purpose of the Study:

  • To develop an integrated on-chip method for sample enrichment, fluorescence labeling, and purification.
  • To demonstrate the utility of this method for quantitative analysis in microfluidic systems.

Main Methods:

  • Fabrication of polymer monoliths (butyl methacrylate) in cyclic olefin copolymer microdevices for solid-phase extraction.
  • On-chip retention, fluorescence labeling (Alexa Fluor 488, Chromeo P503), and elution of analytes.
  • Differential purification based on protein and label retention.

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Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

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Last Updated: May 18, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Main Results:

  • Successful retention, labeling, and elution of fluorophores, amino acids, and proteins.
  • Demonstrated linear correlation between eluted peak areas and concentrations for heat shock protein 90.
  • Achieved simultaneous concentration and labeling with purification on-chip.

Conclusions:

  • The developed method offers a fast, simple, and automatable approach for sample preparation in microfluidics.
  • This technique is compatible with miniaturization and suitable for automated quantitative analysis.
  • The integrated approach enhances efficiency and accuracy in micro total analysis systems.