Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycolipids as host resistance stimulators.

M M Ponpipom1, W K Hagmann, L A O'Grady

  • 1Merck Sharp & Dohme Research Laboratories, Rahway, New Jersey 07065.

Journal of Medicinal Chemistry
|February 1, 1990
PubMed
Summary

6-(5-Cholesten-3 beta-yloxy)hexyl 1-thio-beta-D-mannopyranoside (L-644,257) boosts mouse immunity against Pseudomonas aeruginosa. This compound shows dose-dependent protection via injection, with structural modifications preserving its efficacy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patents and literature.

Applied biochemistry and biotechnology·2013
Same author

Parasitism of Western Corn Rootworm Larvae and Pupae by Steinernema carpocapsae.

Journal of nematology·2009
Same author

Effects of Crop Residue on the Persistence of Steinernema carpocapsae.

Journal of nematology·2009
Same author

Importance of mechanistic drug metabolism studies in support of drug discovery: A case study with an N -sulfonylated dipeptide VLA-4 antagonist in rats.

Xenobiotica; the fate of foreign compounds in biological systems·2008
Same author

Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases.

Multiple sclerosis (Houndmills, Basingstoke, England)·2007
Same author

Multiple sclerosis: an important role for post-translational modifications of myelin basic protein in pathogenesis.

Molecular & cellular proteomics : MCP·2003

Area of Science:

  • Immunology
  • Microbiology
  • Pharmacology

Background:

  • * Cyclophosphamide treatment in mice compromises natural host resistance.
  • * Pseudomonas aeruginosa poses a significant threat in immunocompromised individuals.

Purpose of the Study:

  • * To evaluate the efficacy of 6-(5-Cholesten-3 beta-yloxy)hexyl 1-thio-beta-D-mannopyranoside (L-644,257) in enhancing host defense against Pseudomonas aeruginosa.
  • * To determine the dose-dependency and route of administration for L-644,257's protective effects.
  • * To investigate the structure-activity relationship of L-644,257, including anomeric configuration and side chain modifications.

Main Methods:

  • * Administration of L-644,257 to cyclophosphamide-treated mice challenged with Pseudomonas aeruginosa.
  • * Assessment of protective activity via different routes: subcutaneous (sc), intramuscular (im), intraperitoneal (ip), and oral.

Related Experiment Videos

  • * Comparison of the efficacy of the beta anomer (L-644,257) with its alpha anomer.
  • * Evaluation of related glycolipids (beta-L-fucose, lactose, beta-D-glucose) and modified steroidal side chains.

    • Main Results:

    • * L-644,257 demonstrated dose-dependent enhancement of host resistance against Pseudomonas aeruginosa.
    • * The compound was effective when administered sc, im, and ip, but not orally.
    • * The beta anomer (L-644,257) provided substantially greater protection than its alpha anomer.
    • * The beta-L-fucose glycolipid showed better efficacy with im and ip administration compared to sc.
    • * Lactose and beta-D-glucose glycolipids exhibited minimal to no protective effect.
    • * Modifications to the 17 beta-steroidal side chain did not significantly reduce protective activity.

    Conclusions:

    • * L-644,257 is a potent enhancer of natural host resistance against Pseudomonas aeruginosa infection in an immunocompromised mouse model.
    • * Route of administration and stereochemistry (beta anomer) are critical for L-644,257's efficacy.
    • * The steroidal side chain offers a potential target for further optimization of this immunomodulatory compound.