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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

Quantitative and sensitive protein detection strategies based on aptamers.

Yoshihito Yoshida¹, Iwao Waga, Katsunori Horii

¹VALWAY Technology Center, NEC Soft Ltd., Tokyo, Japan.

Proteomics. Clinical Applications

|September 22, 2012

Summary

Aptamers, functional nucleic acids, offer sensitive detection via PCR amplification. New immuno-aptamer assays enhance ELISA for high-affinity target recognition in diagnostics.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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Published on: March 21, 2018

Area of Science:

Biotechnology and Molecular Diagnostics
Nucleic Acid Aptamer Technology

Background:

Aptamers are single-stranded DNA or RNA oligonucleotides with high target affinity.
Their selective recognition capabilities drive applications in analysis, diagnostics, and therapeutics.
Aptamers offer advantages over antibodies, including exponential amplification via PCR.

Purpose of the Study:

To review recent advancements in aptamer-based detection systems.
To highlight the development and application of immuno-aptamers for IgG detection.
To discuss novel, highly sensitive aptamer-based detection strategies.

Main Methods:

Summarization of recent literature on aptamer detection systems.
Development of immuno-aptamers targeting mouse and rabbit IgG.
Construction of a novel immuno-aptamer PCR assay based on ELISA.

Main Results:

Demonstration of aptamers' exponential amplification via PCR.
Successful development of immuno-aptamers for IgG detection.
Establishment of a sensitive immuno-aptamer PCR assay for enhanced detection.

Conclusions:

Aptamer-based detection systems offer high sensitivity and specificity.
Immuno-aptamer PCR assays represent a significant advancement in diagnostic technology.
Further development of aptamer sensors and PCR-based assays promises improved analytical capabilities.