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Updated: May 18, 2026

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
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A universal nanoparticle cell secretion capture assay.

Wendy Fitzgerald1, Jean-Charles Grivel

  • 1Section on Intercellular Interactions, Program in Physical Biology, The Eunice Kennedy-Shriver National Institute of Health and Human Development, Bethesda, Maryland 20892, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 22, 2012
PubMed
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Researchers developed a novel assay to identify and isolate cells by detecting their secreted proteins. This noninvasive method uses nanoparticles to capture secreted products on the cell surface, enabling further cell culture.

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Secreted proteins are crucial for intercellular communication, particularly within the immune system.
  • Existing methods lack a universal, noninvasive approach for identifying and isolating cells based on their secreted products.

Purpose of the Study:

  • To develop a universal, noninvasive assay for identifying and isolating cells based on their secreted protein products.
  • To enable the capture and analysis of secreted proteins directly on the surface of producing cells.

Main Methods:

  • Development of heterofunctional nanoparticles functionalized with cell surface-specific and secreted protein-specific antibodies.
  • Targeting nanoparticles to the cell surface to capture secreted proteins.
  • Assessment of cell viability and compatibility with subsequent cell culture and expansion.

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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

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Last Updated: May 18, 2026

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

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Published on: February 14, 2022

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Main Results:

  • Successful development of a novel assay for cell identification and isolation based on secreted proteins.
  • Demonstration that the method is noninvasive and preserves cell viability.
  • Confirmation of compatibility with downstream cell culture and expansion.

Conclusions:

  • The developed nanoparticle-based assay offers a universal and noninvasive solution for identifying and isolating cells by their secreted products.
  • This method facilitates further study and application of specific cell populations without compromising their integrity.