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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

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Related Experiment Video

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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Published on: October 7, 2020

Concise review: Spermatogenesis in an artificial three-dimensional system.

Huleihel Mahmoud¹

¹The Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. huleihel@bgu.ac.il

Stem Cells (Dayton, Ohio)

|September 22, 2012

Summary

This summary is machine-generated.

Complete spermatogenesis in vitro was achieved using a novel 3D soft agar matrix culture system. This breakthrough offers new avenues for studying male germ cell development and its clinical applications.

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Area of Science:

Reproductive Biology
Stem Cell Science
Developmental Biology

Background:

Spermatogonial stem cell culture is crucial for understanding male fertility.
Previous in vitro systems (2D and some 3D) have not achieved complete spermatogenesis.
The natural seminiferous tubule environment is a 3D structure with specific regulatory niches.

Purpose of the Study:

To review the advantages of 3D culture systems for studying complete spermatogenesis.
To discuss the potential of a 3D soft agar matrix for in vitro spermatogenesis.
To explore the expansion of this 3D system to human germ cell development and assess sperm functionality.

Main Methods:

Review of existing 2D and 3D cell culture techniques for spermatogonial stem cells.
Description of a novel 3D soft agar matrix culture system.
Discussion of experimental approaches for human germ cell application and functional assessment.

Main Results:

Achievement of complete in vitro spermatogenesis in mouse testicular germ cells using a 3D soft agar matrix.
Demonstration of the potential of 3D culture to mimic the natural testicular environment.
Identification of key factors and steps for advancing 3D culture systems.

Conclusions:

The 3D soft agar matrix culture system represents a significant advancement for achieving complete in vitro spermatogenesis.
This system holds promise for studying male germ cell development and infertility.
Further research is needed to translate these findings to human germ cell applications and reproductive technologies.