Carcinoma in situ of the testes: diagnosis by aspiration flow cytometry

Carcinoma in situ of the testes has been described as a premalignant state with the potential to progress to invasive carcinoma. A history of testicular carcinoma, cryptorchidism, somatosexual ambiguity and infertility has been identified as a risk factor for carcinoma in situ. A series of 25 infertility patients underwent aspiration biopsy of the testis as part of a study protocol to assess spermatogenesis. Of these patients 1 had a unilateral seminoma. In accordance with the study protocol the contralateral testis was aspirated at radical orchiectomy and the deoxyribonucleic acid histogram generated demonstrated an aneuploid peak. Carcinoma in situ subsequently was noted on tissue biopsy. The discovery of carcinoma in situ cells in this manner highlights the potential of this technique as a diagnostic tool for the screening and followup of men at increased risk for carcinoma in situ.