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Updated: May 18, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
[Brucella suis--a little-known zoonotic agent]
Abstract:
Brucellosis is a rare but serious bacterial zoonosis. Officially, the Czech Republic is among countries that are free from brucellosis in livestock. In the country, sporadic imported human infections may occur, caused by e.g. Brucella melitensis. In wild hare populations, however, rare cases of infection caused by Brucella suis are still observed, potentially threatening humans. The short communication reports two cases of hare brucellosis in Moravia, Czech Republic, and experiences with isolate identification by the MALDI-TOF method.
Insights
Brucellosis in hares, caused by Brucella suis, was identified in Moravia, Czech Republic. This rare bacterial zoonosis highlights potential risks to humans from wildlife infections.
Area of Science:
- Veterinary Microbiology
- Zoonotic Diseases
- Bacteriology
Context:
- The Czech Republic is officially recognized as free from brucellosis in livestock.
- Sporadic imported human brucellosis cases, often Brucella melitensis, are documented.
- Brucella suis infections have been observed in wild hare populations, posing a potential human health risk.
Purpose:
- To report two cases of Brucella suis infection in hares in Moravia, Czech Republic.
- To describe the identification of Brucella isolates using the MALDI-TOF method.
Summary:
- Two instances of brucellosis in hares were detected in Moravia.
- The causative agent was identified as Brucella suis.
- MALDI-TOF mass spectrometry was employed for bacterial isolate identification.
Impact:
- Highlights the continued presence of Brucella suis in Czech wildlife, specifically hares.
- Underscores the importance of surveillance for zoonotic diseases in wild animal populations.
- Demonstrates the utility of MALDI-TOF for rapid identification of Brucella species in diagnostic settings.
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