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Updated: May 18, 2026
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
Published on: February 15, 2019
[Comparison of transformation of four processed rhubarb aqueous extracts in intestinal bacteria in vitro]
Rui Song1, Yuan Tian, Zunjian Zhang
1Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China. songrui@cpu.edu.cn
Objective:
To compare the metabolic transformation of four processed rhubarb aqueous extracts in rat intestinal bacteria in vitro.
Method:
Rat intestinal bacteria test solution and each of four processed rhubarb aqueous extracts were incubated under anaerobic conditions at 37 degrees C. High-performance liquid chromatography with diode-array detection (HPLC-DAD) and tandem mass spectrometry (HPLC-MS/MS) was used for the qualitative analysis on the components that can be bio-transformed by rat intestinal bacteria as well as the trend of metabolic transformation of each parent compounds according to the changes in chromatographic peak areas in different incubation times.
Result:
Anthraquinones, glucose gallates and naphthalenes glucosides could be bio-transformed by rat intestinal bacteria. Of them, anthraquinones were undoubtedly the most prevalent parent compounds, as 12 out of the 17 metabolites were tentatively assigned as metabolites transformed from anthraquinones. Besides, it was also found that each parent compound in four processed rhubarb extract were diverse from each other with the incubation time.
Conclusion:
The preparations change composition and proportional relationship of ingredients contained in rhubarb and thus impacting their transformation effect in intestinal bacteria.
