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[Pharmacokinetics of Danshen multi-component osmotic pump tablets in Beagle dogs]
Lin Zhou1, Xianying Liu, Jinfeng Zheng
1Beijing University of Chinese Medicine, Beijing 100102, China. zhoulin1211@163.com
Objective:
To study the variation in plasma concentration after oral administration of Danshen multi-component osmotic pump tablets in Beagle dogs with conventional Danshen tablets as the control preparation, in order to assess the sustained release of Danshen multi-component osmotic pump tablets.
Method:
HPLC was adopted for determination, with Shimadzu's ODS-SP column (4.6 mm x 150 mm, 5 microm), flow rate of 1 mL x min(-1), and column temperature of 30 degrees C. Protein in plasma samples of tanshinone II(A) and salvianolic acid B was removed by ethyl acetate extract and acetone precipitation method respectively. Their pharmacokinetic parameters were calculated based on determination results.
Result:
The methodology study on the two constituents with different polarity showed conformity to the requirements. Compared with conventional Danshen tablets, Danshen multi-component osmotic pump tablets showed decrease in Cmax and extension in Tmax after oral administration in Beagle dogs.
Conclusion:
Danshen multi-component osmotic pump tablet has a good sustained-release effect compared with conventional Danshen tablets.
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