Related Experiment Video
Updated: May 18, 2026
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
[Determination of ubenimex in human plasma by HPLC-MS/MS]
1Department of Clinical Pharmacology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To establish a liquid chromatography tandem mass spectrometry method for the determination of ubenimex in human plasma.
Methods:
The essay was conducted with an API 3000 HPLC-MS/MS system consisted of a Ultimate C18 column (50 mm x 4.6 mm, 5 microm). The mobile phase consisted of methanol-water-formic acid (70 : 30 : 0.05, V/V/V) at a flow rate of 0.2 mL/min. Granisetron was used as the internal standard. The sample was extracted by solid phase extraction column and was operated under the multiple reaction monitoring mode using the electrospray ionization technique in positive mode.
Results:
The linear range of ubenimex was 0.4-4000 ng/mL. The limit of quantity was set at 0.4 ng/mL. The within-day and between-day variations were less than 6%.
Conclusion:
This method for the quantitative determination of ubenimex was proved to be accurate, sensitive, selective and convenient and can be applied in the determination of ubenimex in human plasma.
More Related Videos
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
09:04Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods
Published on: September 10, 2018