Human saliva total protein levels by AV17 pigment based analysis: validation, stability and short-term variation
Maddalena Prearo1, Giorgia Antonelli, Rosalba Gatti
1Clinical Biochemistry, Dept. of Medical Diagnostic and Spec. Ther. University of Padova, Italy.
Background:
A colorimetric method based on acid violet pigment, namely AV17, to analyse salivary total protein content, was assessed.
Methods:
Human saliva sample or standard (50 microL) was added to 1.5 mL of AV17 working solution (1 mg/mL in 75 mmol/L sodium chloride and 1.7 mol/L phosphoric acid). Total protein concentration was measured at 546 nm. Salivary total protein of healthy subjects was analyzed.
Results:
The standard protein was Human Serum Albumin and the detection range was 38 mg/L - 900 mg/L with a LOD and LOQ of 26 mg/L and 64 mg/L, respectively. Intraday CVs were 3% - 5% and interday CVs were 3%-6%. The dilution test demonstrated a correlation coefficient of 0.999 and the recovery tests ranged from 108% to 111%. Saliva sample stability was also demonstrated. No intra-individual salivary total protein variation was found during the morning.
Conclusions:
The method suitability for laboratory diagnostic purposes to analyse human saliva protein content and stability was demonstrated.
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