Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glassware Calibration01:11

Glassware Calibration

Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adult reference values for spirometry, body plethysmography and diffusing capacity adjusted for sex, age, weight, height or arm span - the Danish lung function material (DALFUMAT) study.

European clinical respiratory journal·2026
Same author

Running economy and lower-limb anthropometry in adult male Kenyan and Danish middle- and long-distance runners and in untrained adolescents.

Experimental physiology·2025
Same author

Childhood body size and risk of chronic obstructive pulmonary disease in adulthood: a prospective cohort study.

Respiratory medicine·2025
Same author

Calibration of bronchial methacholine challenge: addressing dose accuracy and practicality.

European clinical respiratory journal·2025
Same author

Increasing prevalence of galactose-α-1,3-galactose sensitization in the Danish general adult population.

Allergy·2024
Same author

Maximal respiratory pressure after COVID-19 compared with reference material in healthy adults: A prospective cohort study (The SECURe study).

Physiological reports·2024
Same journal

Vitamin K-dependent proteins in patients undergoing head and neck free flap surgery - a prospective observational study.

Scandinavian journal of clinical and laboratory investigation·2026
Same journal

Challenges in the interpretation of amphetamine results: differences of reactivity between immunoassay and immunochromatography.

Scandinavian journal of clinical and laboratory investigation·2026
Same journal

Maternal whole blood mercury, cadmium and lead concentrations during pregnancy and lactation and infant heavy metal status at six months.

Scandinavian journal of clinical and laboratory investigation·2026
Same journal

Performance of Sysmex SP-50 automated slidemakers and stainers in three hematology laboratories.

Scandinavian journal of clinical and laboratory investigation·2026
Same journal

Digital misinformation and parental resistance to newborn heel-prick screening: an Instagram content analysis.

Scandinavian journal of clinical and laboratory investigation·2026
Same journal

Serum Krebs von den Lungen-6 and interstitial lung disease: a brief review.

Scandinavian journal of clinical and laboratory investigation·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis
07:27

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis

Published on: February 24, 2023

Validation of spirometer calibration syringes.

Flemming Madsen1

  • 1Allergy and Lung Clinic Helsingør, Lung Function Laboratory, Helsingør, Denmark. fl em-mad@dadlnet.dk

Scandinavian Journal of Clinical and Laboratory Investigation
|September 25, 2012
PubMed
Summary
This summary is machine-generated.

Pulmonary function testing relies on volume calibration syringes, which were validated for accuracy. Most syringes met claimed volumes, demonstrating stable performance but requiring annual calibration for compliance.

More Related Videos

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography
08:51

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography

Published on: August 12, 2014

Related Experiment Videos

Last Updated: May 18, 2026

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis
07:27

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis

Published on: February 24, 2023

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography
08:51

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography

Published on: August 12, 2014

Area of Science:

  • Pulmonary Function Testing
  • Metrology
  • Medical Device Calibration

Background:

  • Volume calibration syringes are critical instruments in pulmonary function laboratories.
  • No published validation data exists for these essential devices.
  • Ensuring accurate calibration is vital for reliable pulmonary function test (PFT) results.

Purpose of the Study:

  • To validate the accuracy of commonly used volume calibration syringes.
  • To establish a traceable calibration chain from certified weights to gas volumes.
  • To assess the long-term stability and accuracy of these critical PFT instruments.

Main Methods:

  • Gravimetric analysis: Syringes were weighed before and after dispensing a known volume of water.
  • Gas volume determination: A modified rolling seal spirometer was used to measure the gas volume corresponding to the dispensed water.
  • Calibration chain establishment: Linked certified water weights to gas volumes for traceability.

Main Results:

  • The volume of spirometer calibration syringes could be verified with an accuracy of ± 15 ml.
  • The modified rolling seal spirometer demonstrated high accuracy (± 11 ml at 1 L, ± 13 ml at 3 L).
  • All validated syringes, except two small 1-L models, met their label claimed volume within ± 0.5%.

Conclusions:

  • Volume calibration syringes exhibit stable stroke volumes over time, even after prolonged use and storage.
  • Annual calibration of spirometer calibration syringes is necessary to meet international standards.
  • Validated calibration methods ensure the accuracy and reliability of pulmonary function measurements.