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High-throughput Screening for Protein-based Inheritance in S. cerevisiae
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[PSI(+) ] prion variant establishment in yeast.

Jaya Sharma1, Susan W Liebman

  • 1Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.

Molecular Microbiology
|September 25, 2012
PubMed
Summary

Yeast prions, or variants, can develop multiple conformations within a single cell. Some cells indefinitely maintain diverse prion variants, suggesting a maturation phase before a single conformation is established.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Yeast Genetics

Background:

  • Mammalian prion diseases exhibit distinct clinical pathologies due to heritable conformations of aggregated PrP proteins, known as prion strains.
  • The yeast [PSI(+) ] prion, mediated by the Sup35 protein, serves as a model for studying prion strain establishment.
  • Transient overexpression of Sup35, particularly in the presence of the [PIN(+) ] prion, can induce diverse prion variant conformations.

Purpose of the Study:

  • To investigate the de novo establishment of prion strains, termed variants, in the yeast model system.
  • To determine if a single cell can harbor and propagate multiple prion variants simultaneously.
  • To explore the potential maturation process of newly formed prions before they stabilize into a specific variant conformation.

Main Methods:

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  • Utilizing the yeast [PSI(+) ] prion model with Sup35 protein overexpression.
  • Employing the [PIN(+) ] prion to facilitate the induction of new [PSI(+) ] variants.
  • Tracking the segregation of prion variants within cell pedigrees over multiple generations.

Main Results:

  • Transient overexpression of Sup35 can induce multiple prion variants within a single yeast cell.
  • While most cells stabilize to a single variant phenotype, approximately 5% of pedigrees indefinitely segregated multiple [PSI(+) ] variants.
  • This suggests that newly formed prions may undergo a maturation phase, allowing for the coexistence and potential diversification of variants.

Conclusions:

  • The yeast prion system demonstrates that multiple prion variants can arise and coexist within a single cell.
  • The findings support a model where newly formed prions mature over time, potentially explaining the establishment and adaptation of prion strains.
  • These insights into yeast prion variant dynamics may offer relevant perspectives on the mechanisms underlying mammalian PrP prion strain establishment and adaptation.