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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Replication of hepatitis C virus genotype 3a in cultured cells
Mohsan Saeed1, Claire Gondeau, Susu Hmwe
1Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Abstract:
Hepatitis C virus (HCV) genotype 3a is widespread worldwide, but no replication system exists for its study. We describe a subgenomic replicon system for HCV genotype 3a. We determined the consensus sequence of an HCV genome isolated from a patient, and constructed a subgenomic replicon using this clone. The replicon was transfected into HuH-7 cells and RNA replication was confirmed. We identified cell culture-adaptive mutations that increased colony formation multiple-fold. We have therefore established a genotype 3a replicon system that can be used to study this HCV genotype.
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