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Related Experiment Video

Updated: May 18, 2026

Quantification of Coenzyme A in Cells and Tissues

Quantification of Coenzyme A in Cells and Tissues

Published on: September 27, 2019

Acetoacetyl-CoA synthetase is essential for normal neuronal development.

Shinya Hasegawa¹, Hiroki Kume, Sayuri Iinuma

¹Department of Health Chemistry, Hoshi University, Ebara, Shinagawa, Tokyo 142-8501, Japan. s-hasegawa@hoshi.ac.jp

Biochemical and Biophysical Research Communications

|September 25, 2012

Summary

Acetoacetyl-CoA synthetase (AACS) is crucial for neuronal development. Its regulation by SREBP-2 and role in cholesterol synthesis are vital for neurogenesis and neuron differentiation.

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Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Cholesterol and fatty acids are vital for neuronal tissue.
Acetoacetyl-CoA synthetase (AACS) utilizes ketone bodies for synthesizing these essential lipids and is highly expressed in the brain.

Purpose of the Study:

To investigate the regulation of AACS during neurite outgrowth.
To clarify the physiological role of AACS in neurogenesis.

Main Methods:

Neuro-2a cell culture and neurite outgrowth induction.
Messenger RNA and protein expression analysis.
Chromatin immunoprecipitation (ChIP) assays.
SREBP-2 knockdown experiments.
Primary neuron culture and differentiation marker analysis.

Main Results:

AACS expression and mRNA levels increased during neurite outgrowth in Neuro-2a cells.
SREBP-2, a key transcription factor for cholesterol synthesis, was found to interact with the AACS promoter, and its knockdown reduced AACS mRNA levels.
AACS expression significantly increased in embryonic mouse brains during late development (E16.5-E18.5).
Knockdown of AACS in primary neurons led to decreased expression of neuronal differentiation markers (MAP-2, NeuN) and synaptopodin.

Conclusions:

AACS expression is regulated by SREBP-2.
AACS plays a significant role in normal neuronal development, including differentiation and synapse formation.